The Regulatory Effects of Purα on PARP1 Gene Expression and its functions on DNA repair

  • Wenxin He He Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
  • Jianqi Cui Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
  • Yongling Li Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China)
  • Xiaoguang Shi Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
  • Juan Chai Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
  • Binying Zhang 2. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China)
  • Shanshan Guo Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
  • Chengmin Yuan Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
  • Ping Li Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China)
  • Tao Sun Ningxia Key Laboratory of Cerebrocranial Diseases，The Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, 750004, China
DOI: https://doi.org/10.36013/jimsa.v1i1.8
Keywords: Purα; poly ADP-ribose polymerase 1; regulation of gene expression, DNA damage and repair

Abstract

Abstract: The luciferase reporter construct, which contains a poly ADP-ribose polymerase 1 gene promoter, was transfected into U87MG cells with the Purα eukaryotic expression vector, and the activities of PARP1 promoter were assessed by luciferase assay to evaluate the regulatory effects of Purα on PARP1 gene expression. The Purα eukaryotic expression vector was transfected into U87MG cells, and the cell total RNA and protein were extracted to determine the effects of Purα on PARP1 gene expression at transcriptional and translational levels by real-time PCR and Western blotting assay. The results demonstrated that Purα can positively regulate PARP1 promoter activity and promote PARP1 gene expression both at transcriptional and translational levels. The further study illustrated that Purα can collaborate with PARP1 in the repair of damaged DNA; the results of a pull-down assay suggested that there is a physical interaction between Purα and PARP1. The overexpression of Purα can increase endogenous PARP1 expression and alleviate the expression of the DNA damage signal protein γH2AX. Above all, we believe that Purα possesses a positive regulatory effect on PARP1 gene expression and collaborates with PARP1 to repair damaged DNA.

Keywords: Purα; poly ADP-ribose polymerase 1; regulation of gene expression, DNA damage, and repair.

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Published
2020-08-16
How to Cite
He, W. H., Cui, J., Li, Y., Shi, X., Chai, J., Zhang, B., Guo, S., Yuan, C., Li, P., & Sun, T. (2020). The Regulatory Effects of Purα on PARP1 Gene Expression and its functions on DNA repair. Journal of Internal Medicine: Science & Art, 1(1), 22-31. https://doi.org/10.36013/jimsa.v1i1.8
Issue
Vol. 1 (2020)
Section
Articles

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